With a wall thickness of 1.25 mm, the standard outer dimensions of a bucket are 12.5 x 12.5 m, height 45 mm. This consideration is especially important for buckets that are designed to measure small volumes and therefore have very small measuring windows. The selection of different types of buckets is more than expected, even those buckets used for absorption measurements in the field of UV-Vis spectroscopy.
Buckets are usually made of various transparent materials, such as optical glass, quartz, sapphire or optical plastic. Each material has unique translucent properties and it is important to know these characteristic optical properties before selecting the baking material. If the sample is based on an aqueous solution, then the material from which the bucket is made is relatively insignificant. If, on the other hand, they are organic solvents, glass buckets have the preferred option, as they show greater strength compared to variants of plastic. Organic solvents, on the other hand, glasses and quartz semimers are preferred because they are more robust compared to plastic alternatives. And the NRC will not work with organic solvents, instead it should go with CRF or HTR versions.
The absorption that is in the range of 0.2 A to 1.5 A generates the most accurate results. A micro-volume instrument with a very short path length (1 mm or 0.1 mm) is ideal for highly concentrated samples. Buckets uv vis cuvettes refer to the distance between the inside of the walls of the opposite buckets, representing the length of the trajectory, that light from the spectrometer travels through the sample during measurement.
Our IR quartz semimers provide excellent transmission over the near-infrared, short IR and medium-IR spectra. Fluorescence measurements are usually made by optically “exciting” the same solution at an angle of 90 degrees to the spectrometer system. This requires buckets with optically clear walls 90 degrees apart, and this is usually achieved by making the four walls of the bucket optically clear without “frozen” sides to handle the bucket.
You’re going to pay a little more for quartz, but you also get an extended transmission range of 190-2,500 nm. For UV experiments, you definitely need a UV quartz bucket, and we recommend that you don’t try to cut corners here, because by buying a cheap UV cell, your data won’t be up to par. If you’re on a tight budget, you’ll want an optical glass bucket. This baking material is ideal for working in the VIS range and has a decent transmission range of 340-2,500 nm. Most applications will fall into this range and many do not need the additional UV points obtained with the other materials. Rapid verification of the purity and concentration of RNA and DNA is a particularly widespread application.